Delayed Fluorescence as a Screening Tool |
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A fast and reliable method to measure the activity of photosynthetic herbicides in intact plants |
Analysis of the ResultsDose-response curvesThe dose-response relationship can be obtained by measuring the effect of the herbicide (response) at a set of different concentrations (doses). As a measure of the effect we use the amplitude of the I4 peak of the DFIC. The dose-response curve can be approximated to a sigmoidal function whose upper assymptote corresponds to untreated samples and the lower assymptote to samples with fully inhibited electron transport. The dose-response curve provides a mean to estimate the concentration of half-inhibition, or its negative logarithm - the index of half-inhibition, pI50. The higher pI50 is, the more sensitive is the plant towards the given herbicide (or the more effective is the herbicide in the given plant).
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